Insecticide misuse in tropical rice is beginning to accelerate the development of insecticide resistance. High levels of resistance have been detected in the Mekong Delta (Read: resistance to BPMC, resistance to fipronil, resistance to imidacloprid). There are numerous attempts to measure insect responses to insecticides using a range of bioassay methods. However, unless the methodology used in these detections are quite similar it is difficult to compare their differences. We initiated an insecticide resistance network, developed standardized methods to be used in respective countries to collect a range of LD50 determinations in several countries and trained research partners (Read: Toxicology Report).
The standardized methods, including hands-on procedures in insect collection, rearing, preparation, bioassays and data analyses using the toxicological statistics software POLO plus are now published. The book is entitled “Research Methods in Toxicology and Insecticide Resistance Monitoring of Rice Planthoppers” by K.L. Heong, K.H. Tan, C.P.F. Garcia. L.T. Fabellar and Z. Lu (2011) and free download is available from http://books.irri.org/9789712202605_content.pdf.
Additional information on insecticide use
British couple and five other tourists died in Thailand ‘because of bed bug pesticide poisoning’. The suspected insecticide was chlorpyrifos commonly used in rice fields.