Insecticide resistance is a heritable change in an insect population’s response to a particular biocide. Pest populations with increased resistance to a particular insecticide would mean the need for increased applications or increase in concentrations and in extreme conditions, rendering the active ingredient or groups of active ingredients with similar modes of action (cross resistance) to become non effective. Insecticide resistance can be monitored and researched through field collections, laboratory rearing and the use of bioassay techniques. For such bioassay data to be accurate, scientifically acceptable, and usable in comparisons between regions, production conditions and environments, there is need to use standardized methodologies and analytical procedures, to avoid contaminating the results.
The new edition of the book “Research Methods in Toxicology and insecticide Resistance Monitoring of Rice Planthoppers (2nd Edition)” is released in November 2013. The first edition, which has been out of print since December 2012, is now replaced by a more complete second edition. Four additional chapters of research methods have been included. A chapter focusing on analyzing quantal response data with multiple explanatory variables with the use of PoloEncore© and a chapter describing other forms of dose-response analyses with some examples and the determination and discussion of LC50s and LT50s are new. Another chapter describes insecticide resistance reversion and the use of the resistance stability point as a more stable baseline for comparing resistance in space and time. The use of molecular tools to detect field resistance with examples is described in another chapter. Molecular techniques have some advantages as they are easier to apply and can also detect resistance due to target site mutations sometimes known as the second phase in resistance development. Finally, a chapter describing the evaluation of repellent effects using methyl eugenol as an example is also included. The first seven chapters have been updated and an extended bibliography has been included for readers’ reference. The second edition also includes a CD with the pdfs of the book, sample datasets and graphs.
Free download of the book in pdf is available in Google Books.